Biodegradation of malachite green by a novel laccase-mimicking multicopper BSA-Cu complex: Performance optimization, intermediates identification and artificial neural network modeling

摘要

In this work, a soluble biopolymer was prepared by conjugating the bovine serum albumin (BSA) with transition metal ion (Cu2+). BSA-Cu complex was synthesized and characterized using UV-vis absorption, fluorescence and ATR-FTIR spectroscopies. A colorimetric guaiacol oxidation based method, was used to study the catalytic activity of complex and the results indicated its laccase-like activity. Compared with laccase, BSA-Cu complex showed a higher K-m value and a similar V-max value at the same mass concentration. Also, the ability of the BSA Cu complex to decolorize malachite green (MG) was tested and the results showed that the complex was able to complete the decolorization process of MG within 30 min. Using gas chromatography/mass spectrometry (GC-MS) the resultant metabolites of MG degradation were analyzed and the toxicity of degradation products was assessed against Escherichia coli and Bacillus subtilis. The results confirmed the formation of less toxic products after degradation of MG by BSA-Cu complex. To predict the decolorization efficiency (DE%) of MG, an artificial neural network (ANN) was designed with five, five and one neurons in the input, hidden and output layers, respectively. The obtained results showed the ability of the designed ANN to predict MG removal successfully.