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Molecular analysis of ‘Candidatus Phytoplasma aurantifolia’ associated with phytoplasma diseases in Myanmar

机译:缅甸“ Candidatus phytoplasma aurantifolia”与植物质体疾病相关的分子分析

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摘要

During 2010 and 2011, typical phytoplasma disease symptoms such as little leaves, phyllody and witches’ brooms were observed on black gram, green gram, long bean, shaggy button weed and sesame plants from different regions of Myanmar. The symptomatic samples were analyzed by PCR using universal phytoplasma primers and characterized by sequencing 16S rRNA, ribosomal protein and translocase protein genes. Based on sequence and phylogenetic analysis of the three genes, the phytoplasmas associated with those plants belonged to members of ‘Candidatus Phytoplasma aurantifolia’. To our knowledge, black gram and shaggy button are new hosts for ‘Ca. P. aurantifolia’.
机译:在2010年和2011年期间,在缅甸不同地区的黑克,绿色克,长豆,蓬松的按钮杂草和芝麻植物中观察到典型的植物质体病症状,如小叶,叶状幼虫和女巫的扫帚。对有症状的样品进行PCR,使用通用的植物原质引物进行分析,并对16S rRNA,核糖体蛋白和转位酶蛋白基因进行测序。根据这三个基因的序列和系统发育分析,与这些植物相关的植物原质属于“ Candidatus Phytoplasma aurantifolia”的成员。据我们所知,黑克和蓬松的按钮是‘Ca’的新主机。 P. aurantifolia”。

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