Comparison of Lipids Extracted by Different Methods from Chinese Mitten Crab (Eriocheir sinensis) Hepatopancreas

摘要

The effects of four different extraction methods (Folch, Soxhlet, two-step, and enzyme-assisted aqueous extraction) on the yields, lipid class, fatty acids (FAs) composition, minor components (including carotenoid, cholesterol), and thiobarbituric acid reactive substances values of lipids in the hepatopancreas of Chinese mitten crab (Eriocheir sinensis) were investigated. The C16:0, C18:1, and C18:2 were identified to be the dominant FAs in crab lipids, and the FAs were present in the form of triglycerides. The Soxhlet and enzyme-assisted extraction were more suitable for crab lipids extraction, showing higher extraction rates and oxidative stability. Especially, the lipid extracted by enzyme-assisted extraction has high carotenoids content. The components of crab lipids extracted by enzyme-assisted aqueous extraction were further identified using untargeted metabolomics methods. The polyunsaturated fatty acid, sterols, amino acids, products of lipid β-oxidation and ATP degradation, phosphatidyl ethanolamine, and astaxanthin were founded in crab oil.