Optimization of mannooligosaccharides production from different hydrocolloids via response surface methodology using a recombinant Aspergillus sojae β-mannanase produced in the microparticle-enhanced large-scale stirred tank bioreactor

摘要

In this study, a recombinant Aspergillus sojae beta (β)-mannanase produced from carob extract in the microparticle-enhanced bioreactor was applied to locust bean gum (LBG) and guar gum (GG) as hydrocolloids. The effects of enzyme amount (EA), temperature (T), and gum concentration (GC) on mannooligosaccharides (MOSs) enzymatic fabrication were examined using Box–Behnken response surface methodology. Under optimized conditions (GC = 1%, T = 40°C, EA = 7.5 ml, and incubation time = 10 min for LBG and GC = 0.75%, T = 50°C, EA = 7.5 ml, and incubation time = 35 min for GG), the M2, M3, M4, M5, and M6 fabrications from LBG were 455.2, 801.1, 101.1, 346.9, and 1,592.3 ppm, while the M2, M3, and M6 concentrations from GG were 92.85, 220.99, and 1,882.54 ppm, respectively. The M6 was the most dominant MOS from both gums. Consequently, LBG and GG can be potential sources of MOS production. It has been observed that MOS productions can be enhanced by optimizing β-mannanase enzyme treatment conditions.