Characterization of arsenite-oxidizing bacteria isolated from arsenic-contaminated groundwater of West Bengal

摘要

Nine arsenic (As)-resistant bacterial strains isolated from As-rich groundwater samples of West Bengal were characterized to elucidate their potential in geomicrobial transformation and bioremediation aspects. The 16S rRNA gene-based phylogenetic analysis revealed that the strains were affiliated with genera Actinobacteria, Microbacterium, Pseudomonas and Rhizobium. The strains exhibited high resistance to As [Minimum inhibitory concentration (MIC) ≥ 10 mM As~(3+) and MIC ≥ 450 mM As~(5+)] and other heavy metals, e.g., Cu~(2+), Cr~(2+), Ni~(2+), etc. (MIC ≥ 2 mM) as well as As transformation (As~(3+) oxidation and As~(5+) reduction) capabilities. Their ability to utilize diverse carbon source(s) including hydrocarbons and different alternative electron acceptor(s) (As~(5+), SO_4~(2-), S_2O_3~(2-), etc.) during anaerobic growth was noted. Growth at wide range of pH, temperature and salinity, production of siderophore and biorilm were observed. Together with these, growth pattern and transformation kinetics indicated a high As~(3+) oxidation activity of the isolates Rhizobium sp. CAS934i, Microbacterium sp. CAS905i and Pseudomonas sp. CAS912i. A positive relation between high As~(3+) resistance and As~(3+) oxidation and the supportive role of As~(3+) in bacterial growth was noted. The results highlighted As~(3+) oxidation process and metabolic repertory of strains indigenous to contaminated groundwater and indicates their potential in As~(3+) detoxification. Thus, such metabolically well equipped bacterial strains with highest As~(3+) oxidation activities may be used for bioremediation of As contaminated water and effluents in the near future.