UNMODIFIED EXPRESSION OF rbcS AND rbcL GENES MAY BE RESPONSIBLE FOR THE RESISTANCE OF ALBANIAN LOCAL WHEAT CULTIVARS DAJTI, LVS AND PROGRES, TOWARD MEDITERRANEAN HIGH TEMPERATURES

摘要

In this study were evaluated the expression of rubisco genes (rbcS and rbcL) from three local wheat cultivars of Albania, named Dajti, LVS and Progres, in order to elucidate molecular factors responsible for their resistance toward Mediterranean high temperatures. Seeds donated from the Centre for Genetic Resources of the Agricultural University of Tirana were germinated in culture following a temperature stress regime, and gene expression was controlled at vegetative and anthesis phase. For this, total ARN was extracted from leaves and RT-PCR was developed using primers specific for rubisco genes following the instructions of HS RT-PCR-SIGMA Aldrich Kit. The electrophoresis of the RT-PCR products was done in 1.4% agarose gel and evaluation of gene expression rate was done based on the concentration of the product in gel. Results show that rbcS and rbcL coding genes were expressed from the three wheat cultivars under stress conditions; Modification of stress regime from 25 to 30 degrees C to 35 degrees C did not affect the expression rate at both phase's. In conclusion, at three local wheat cultivars there is not suppression of the expression of rubisco genes, however research should be continued in order to verify the synthesis of Rubisco activase, which can modify the catalytic efficiency of rubisco even though it is produced normally under specific stress conditions.