Utilization of agroindustrial wastes for the production of laccase by Achromobacter xylosoxidans HWN16 and Bordetella bronchiseptica HSO16

摘要

Agroindustrial residual lignocellulosic biomaterial provides an economical and renewable natural bioresource for the large-scale, gainful biofuel production, as well as the production of fine bulk chemicals, which may include industrial biocatalysts. To this end, the laccase-inducing aptitude of some agroindustrial, lignocellulosic residues were appraised in submerged fermentation batch culture of two woodland betaproteobacteria (Hb9c; Achromobacter xylosoxidans HWN16, Hb16c; Bordetella bronchiseptica HSO16). Significant fermentation factors for laccase production were identified following a one-variable-at-a-time: OVAT screening method, levels of significant factors were optimized using response surface methodology: RSM. Mandarin peelings: MP and wheat bran: WB were suitable substrates for laccase production in Hb9c; 29.4 U/mL and Hb16c; 28.2 U/mL, respectively. However, the numerical optimization of significant factors for laccase production in both isolates presented an overall maximum laccase output encountered throughout the study (Hb9c; 169.39 U/mL, Hb16c; 45.22 U/mL), albeit the simulated conditions of the statistical model were outside the design space of the algorithm such as pH 5, 0.5 g MP, 100 rpm, 0.25 g NaNO3 for Hb9c and pH 3, 2.5 g WB, 50 rpm, 0.05 g yeast extract for Hbl6c. Furthermore, a record 17.5 and 15.54 fold increase in laccase turnover depicts the astuteness of the statistical method in the valorization of these lignocellulosic residues for enhanced laccase production, hence, the incorporation of these outcomes at industrial scales might yield tremendous outputs.