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首页> 外文期刊>Journal of environmental biology >Biodegradation of anionic surfactant, sodium dodecyl sulphate by Pseudomonas aeruginosa MTCC10311
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Biodegradation of anionic surfactant, sodium dodecyl sulphate by Pseudomonas aeruginosa MTCC10311

机译:铜绿假单胞菌MTCC10311对阴离子表面活性剂十二烷基硫酸钠的生物降解

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摘要

The anionic surfactant sodium dodecyl sulphate (SDS), the core components of detergent and cosmetic product formulations, contributes significantly to the pollution profile of sewage and wastewater of all kinds. In this study, 44 SDS degrading strains were isolated by soil enrichment methods and the utilization efficiency was assessed by methylene blue active substances (MBAS) assay and High performance liquid chromatography (HPLC) method. Isolate S2 which showed maximum degradation was identified as Pseudomonas aeruginosa MTCC 10311 based on phenotypic features and 16 S rDNA typing .The isolate was found to harbor plasmid within the size range of 9-10 kb. The cured derivative of SDS degrading Pseudomonas aeruginosa was obtained at a frequency of 10.7% by incubation with ethidium bromide (500 mg ml~-1) at 40℃. 96% of SDS degradation occurred at 1500 ppm level within 48 hr of incubation, whereas higher concentration of SDS (10000 ppm) showed only 20% degradation. The optimum temperature and pH was 30℃ and 7.5, respectively. The additional supplementation of carbon and nitrogen source increased the degradation capacity from 93 to 95% and 90 to 96% respectively within 36 hrs of incubation.
机译:阴离子表面活性剂十二烷基硫酸钠(SDS)是洗涤剂和化妆品配方的核心成分,对各种污水和废水的污染状况有很大贡献。本研究通过土壤富集方法分离了44株SDS降解菌,并通过亚甲基蓝活性物质(MBAS)法和高效液相色谱(HPLC)法评价了其利用效率。根据表型特征和16 S rDNA分型,将表现出最大降解的分离株S2鉴定为铜绿假单胞菌MTCC 10311,发现该分离株具有9-10 kb大小的质粒。通过与溴化乙锭(500 mg ml〜-1)在40℃温育,以10.7%的频率获得降解SDS的降解铜绿假单胞菌的固化衍生物。 96%的SDS降解在孵育48小时内以1500 ppm的水平发生,而更高浓度的SDS(10000 ppm)仅显示20%的降解。最佳温度和pH分别为30℃和7.5。在培养的36小时内,碳和氮源的额外补充分别将降解能力从93%提升至95%,将90%提升至96%。

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