Rapid Simultaneous Determination of Organic Acids, Free Amino Acids, and Lactose in Cheese by Capillary Electrophoresis

摘要

A capillary electrophoresis (CE) method for the is- multaneous separation of 11 metabolically important organic acids (oxalic, formic, citric, succinic, orotic, uric, acetic, pyruvic, propionic, lactic, and butyric), 10 amino acids (Asp, Glu, Tyr, Gly, Ala, Ser, Leu, Phe, Lys, and Trp), and lactose has been optimized, validated, and Tested in dairy products. Repeatability and linearity Were calculated for each compound, with detection milt Values as low as 0.2·10~-2 mM for citric acid and Gly. The method as applied to analyze yogurt and different Varieties of commercial cheeses.