Reproduction

摘要

Diets fed during the transition period play an important role in the cow’sreproductive tract following parturition. We aimed to determine the effectsof feeding rumen-protected Lys (RPL, AjiPro-L Generation 3, AjinomotoHeartland Inc., Chicago, IL) pre- (0.54%DM of TMR) and/or postpartum(0.395%DM of TMR) on uterine health and follicular dynamics of Holsteincows (n = 75). Treatments began at 28d before expected calving andconsisted of TMR with RPL pre- and postpartum (LL), with RPL pre- andwithout RPL postpartum (LC), without RPL pre- and with RPL postpartum(CL), and without RPL pre- and postpartum (CC). Uterine health wasevaluated at 4, 7, 10, 13, 15, and 17 DRC by evaluating purulent vaginaldischarge (PVD) via Metricheck (MC) Score and MC Smell. Swabs ofthe endometrium were collected at 15 and 28 DRC. Polymorphonuclearleukocytes (PMN) were evaluated as a percentage of the epithelial cells.Follicular development was monitored via ultrasound every 2 d starting at7 DRC until 28 DRC. Data were analyzed using MIXED and GLIMMIXprocedures in SAS. Cows in C (20.39 ± 3.49%) had greater (P < 0.01)proportion of PMN cells in the uterus than cows in L (14.02 ± 3.49%); andthere was also an effect of POS on PMN, in which cows in LC (18.88 ±3.49%) had greater (P < 0.01) postpartum proportion of PMN cells in theuterus than cows in LL (9.16 ± 3.49%). A PRE × POS interaction for PMNwas present, as cows in LL (9.16 ± 3.49%) had lower (P < 0.01) PMNproportion than cows in LC (18.88 ± 3.49%), CL (20.50 ± 3.49%), and CC(20.76 ± 3.49%). Cows in CL were more likely to develop PVD than cowsin LL (95CI = 2.08–61.91, P = 0.01) or LC (95CI = 1.33–23.55, P = 0.02)at 13 DRC. A POS effect (P = 0.02) on the total growth of the first dominantfollicle (DF) was detected, in which cows in LC (11.19 ± 1.68mm)and CC (11.34 ± 1.68mm) had greater total growth of DF than cows inLL (6.32 ± 1.68mm) and CL (7.73 ± 1.68mm). In conclusion, feedingcows with RPL pre- and postpartum improved uterine health postpartum,and the inclusion of RPL postpartum decreased growth of the first DF.