Short communication: Complete genome sequence of Lactobacillus plantarum J26, a probiotic strain with immunomodulatory activity

摘要

Lactobacillus plantarum J26, a significant probioticisolated from Chinese traditional fermented dairy products,exerts a positive immunomodulatory effect byregulating the expression of immune-related genes. Weinvestigated expression of the cytokines IL-1α, IL-1β,IL-6, and tumor necrosis factor-α in the intestinal tractof mice stimulated by L. plantarum J26. In vivo, thesecytokines were upregulated, peaked on d 5, and then decreasedto the control level, indicating that L. plantarumJ26 could induce expression of the genes encoding theseproinflammatory cytokines. Teichoic acids produced byL. plantarum are recognized as key immunomodulatorymolecules involved in the regulation of the host immuneresponse. To better understand the genetic basis of thisimmunomodulatory mechanism, we sequenced andanalyzed the whole genome of L. plantarum J26. Thegenome of L. plantarum J26 contains a circular chromosomeand 4 circular plasmids. Lactobacillus plantarumJ26 was predicted to synthesize ribitol-type backbonesof wall teichoic acid. Furthermore, orthologous averagenucleotide identity (OrthoANI) values showed that thegenome was highly similar (＞98.00%) to other L. plantarumstrains, especially to L. plantarum ST-III andJDM1. The genomic data of L. plantarum J26 providea genetic basis to further elucidate its mechanism ofimmunoregulation and will facilitate its application inthe functional dairy food industry.