biodegradation during contaminant transport in porous media: 4. Impact of microbial lag and bacterial cell growth

摘要

Miscible-displacement experiments were conducted to examined the impact of microbial lag and bacterial cell growth on the transport of salicylate, a model hydrocarbon compound. The impacts of these processes were examined separately, as well as jointly, to determine their relative effects on biodegradation dynamics. For each experiment, a column was packed with porous medium that was first inoculated with bacteria that contained the NAH plasmid encoding genes for the degradation of naphthalene and salicylate, and then subjected to a step input of salicylate solution. The transport behavior of salicylate was non-steady for all cases examined, and was clearly Influenced by a delay (lag) in the onset of biodegradation.