Rapid detection of the factor V Leiden (1691G>A) and haemochromatosis (845G>A) mutation by fluorescence resonance energy transfer (FRET) and real time PCR

摘要

A rapid methods based on fluorescence resonance energy transfer (FRET) and real time polymerase chain reaction (PCR) was used to identify the haemo- chromatosis genotype in 112 individuals and the factor V genotype in 134 individu- als. The results were compared with conventional methods based on restric- tion enzyme digestion of PCR products.