Efficient utilization of waste paper as an inductive feedstock for simultaneous production of cellulase and xylanase by Trichoderma longiflorum

摘要

Waste paper, one of the major components of municipal and industrial wastes, has considerable potential as a low-cost inductive feedstock for enzyme production. In this study, four kinds of waste papers were used for simultaneous production of cellulase and xylanase by Trichoderma longiflorum. The highest FPase activities (3.21 IU/mL and 2.97 IU/mL) were achieved using waste office paper (WOP) and corrugated board (CB), respectively, followed by magazine paper (MP, 2.51 IU/mL) and tissue paper (TP, 2.34 IU/mL). The maximum beta-glucosidase activities (0.51 IU/mL) and xylanase activities (382.59 IU/mL) were obtained from CB substrates, which were higher than that in the reported literature using waste paper. Correspondingly, the soluble protein concentrations and SDS-PAGE profiles from CB fermentation revealed that more enzymes were secreted during fermentation compared with other waste paper. Moreover, the structural features of different waste paper were characterized by using SEM and XRD technique and revealed that the structural variations were mainly caused by the raw wood sources and papermaking processes, and further affected the fermentation capacity. In addition, a higher beta-glucosidase activity of 0.80 IU/mL were obtained from the mixed carbon source of CB and wheat bran (WB), which were significantly improved and increased by 56.86% when compared with the CB as single carbon source. This study confirmed that the waste paper without pretreatment could be efficiently utilized as an inductive feedstock for simultaneous production of cellulase and xylanase by T. longiflorum. Therefore, these findings obtained from this study will provide a more widely insight into investigating the low-cost inductive feedstock for enzyme production, which were of great benefit both for the economy and environment.