首页> 外文期刊>Journal of Artificial Organs >Impact of 3,4-dideoxyglucosone-3-ene (3,4-DGE) on cytotoxicity of acidic heat-sterilized peritoneal dialysis fluid
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Impact of 3,4-dideoxyglucosone-3-ene (3,4-DGE) on cytotoxicity of acidic heat-sterilized peritoneal dialysis fluid

机译:3,4-dideoxyglucosone-3-ene(3,4-DGE)对酸性热灭菌腹膜透析液细胞毒性的影响

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Of the glucose degradation products (GDPs) in glucose-rich peritoneal dialysate, we investigated the influence of 3,4-dideoxyglucosone-3-ene (3,4-DGE) on the cytotoxicity of acidic heat-sterilized peritoneal dialysis fluid (L-H PDF) using human peritoneal mesothelial cells (HPMC). We prepared acidified filtration-sterilized PDF (glucose concentration 3.86%) containing eight types of added GDP [3,4-DGE, glyoxal (GO), methylglyoxal (MGO), 3-deoxyglucosone (3-DG), formaldehyde (FA), acetaldehyde (AA), 5-hydroxymethyl-2-furaldehyde (5-HMF), and furfural (FF)] or seven types of GDP (GO, MGO, 3-DG, FA, AA, 5-HMF, and FF). HPMC were exposed to these two types of solution and acidic heat-sterilized PDF (glucose concentration 3.86%, L-H 3.86) for 4 h. Cell viability was determined by 3,(4,5-dimethythiazol-2-yl)2,5-diphenyl-terazolium bromide (MTT) assay. MTT viability was decreased significantly compared with the control when treated with L-H 3.86 or acidified neutral filtration-sterilized PDF (glucose concentration 3.86%) containing eight GDPs. However, no significant decrease in MTT viability was observed when HPMC were treated with acidified neutral filtration-sterilized PDF (glucose concentration 3.86%) containing seven GDPs. Thus, 3,4-DGE strongly affects the cytotoxicity of L-H PDF. It is suggested that the cytotoxicity of L-H PDF is based on the presence of 3,4-DGE.
机译:在富含葡萄糖的腹膜透析液中的葡萄糖降解产物(GDPs)中,我们研究了3,4-dideoxyglucosone-3-ene(3,4-DGE)对酸性热灭菌腹膜透析液的细胞毒性的影响(LH PDF )使用人类腹膜间皮细胞(HPMC)。我们制备了酸化的过滤灭菌的PDF(葡萄糖浓度为3.86%),其中包含八种添加的GDP [3,4-DGE,乙二醛(GO),甲基乙二醛(MGO),3-脱氧葡糖酮(3-DG),甲醛(FA),乙醛(AA),5-羟甲基-2-糠醛(5-HMF)和糠醛(FF)]或七种GDP(GO,MGO,3-DG,FA,AA,5-HMF和FF)。 HPMC暴露于这两种类型的溶液和酸性热灭菌的PDF(葡萄糖浓度为3.86%,L-H为3.86)持续4 h。细胞存活力通过3,(4,5-二甲基噻唑-2-基)2,5-二苯基溴化唑鎓(MTT)测定来确定。当用L-H 3.86或酸化的中性过滤灭菌的PDF(葡萄糖浓度为3.86%)处理时,MTT活力与对照相比显着降低,葡萄糖含量为3.86%。但是,当HPMC用含有7个GDP的酸化中性过滤灭菌PDF(葡萄糖浓度为3.86%)处理时,未观察到MTT活力的显着降低。因此,3,4-DGE强烈影响L-H PDF的细胞毒性。提示L-H PDF的细胞毒性是基于3,4-DGE的存在。

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