Application of a Nitrospiropyran-FAD-Reconstituted Glucose Oxidase and Charged Electron Mediators as Optobioelectronic Assemblies for the Amperometric Transduction of Recorded Optical Signals: Control of the 'On'-'Off' Direction of the Photoswitch

摘要

The directionality of the enzyme photoswitch is controlled by the affinities of the oxidized electron mediators to diffusionally penetrate the protein nad reach appropriate electron transfer distances to oxidize the FADH_2 redox center of the enzyme. In all of the systems, cyclic amperometric transduction of optical signals recorded by the photoisomerizable enzyme is accomplished. The enzyme photoisomer "On"-"Off" switching direction is controlled by tuning the electrical interactions of the electron mediator and the photoisomerizable enzyme.