Pulse duration determines levels of Hsp70 induction in tissues following laser irradiation

摘要

Induction of heat shock protein (Hsp) expression correlates with cytoprotection, reduced tissue damage,nand accelerated healing in animal models. Since Hsps are transcriptionally activated in response to stress, theyncan act as stress indicators in burn injury or surgical procedures that produce heat and thermal change. A fastnin vivo readout for induction of Hsp transcription in tissues would allow for the study of these proteins asntherapeutic effect mediators and reporters of thermal stress/damage. We used a transgenic reporter mouse innwhich a luciferase expression is controlled by the regulatory region of the inducible 70 kilodalton (kDa) Hsp as anrapid readout of cellular responses to laser-mediated thermal stress/injury in mouse skin. We assessed the pulsenduration dependence of the Hsp70 expression after irradiation with a CO2 laser at 10.6 μm in wavelength over anrange of 1000 to 1 ms. Hsp70 induction varied with changes in laser pulse durations and radiant exposures, whichndefined the ranges at which thermal activation of Hsp70 can be used to protect cells from subsequent stress, andnreveals the window of thermal stress that tissues can endure. C u0002 2011 Society of Photo-Optical Instrumentation Engineers (SPIE).n[DOI: 10.1117/1.3600013]