Isolation And Characterization Of Bifenthrin and Cypermethrin Tolerant Bacteria From Pesticide Contaminated Soil From Punjab

摘要

Pesticides contaminated soil is the most appropriate ecological niche which gives rise to such autochthonous microbial populations having the capacity to resist or degrade the particular class of pesticides. Isolation of indigenous bacterial strains having the potential of degrading pyrethroids insecticides has acknowledged significant consideration throughout the world due to their environment friendly in situ bioremediation capability. For isolation of bifenthrin and cypermethrin resistant bacterial strains from pesticides contaminated soils, enrichment culture technique was used. The isolated bacteria were further screened based on their morphological characteristics, biochemical parameters and pesticides tolerant capacity. Furthermore, the effect of different growth factors, like temperature, pH were also studied. In the present study, three bacterial strains were isolated having the ability to tolerate cypermethrin upto the concentration of 150 μg/mL. Molecular and phylogenetic analysis of 16S rRNA gene of bacteria identified them to be Pseudomonas aeruginosa, Pseudomonas monteilii and Alcaligenes faecalis. Pseudomonas monteilii was found to be the most tolerant strain against pesticide cypermethrin upto the concentration of 200 μg/mL. Pseudomonas monteilii and Bacillus licheniformis were found to be most resistant towards pesticides bifenthrin upto the concentration of 150 μg/mL and 200 μg/mL, respectively. These indigenous bacterial strains can be used in bioremediation of pesticide contaminated soil in situ.