Potential Metabolites Of Bacillus subtilis Strain Isolated From Rhizospheric Soils Of Revegetated Mine Spoil Dumps

摘要

Identification of plant growth promoting rhizobacteria (PGPR) to be used as a bio-inoculum during the revegetation process of mine spoil dumps becomes necessary since they can aid in the successful establishment of the plants which leads to healthy plant microbe interaction in the rhizosphere. In this study, the potential metabolites produced by a predominant PGPR strain of Bacillus subtilis isolated from Leucaena leucocephala (C.N- Subabul family-Leguminaosae) from lignite mines of Neyveli, Tamil Nadu were assessed. In addition to the production of indole acetic acid (41 μg/mL) and gibberellic acid (75.15 μg/mL) in substantial amounts, the organism was able to produce phytochemical constituents, like phenols, proteins, amino acids and various other secondary metabolites as revealed by FTIR, HPLC and GC-MS analysis. The study of the contribution of rhizobacteria in stabilizing the plant microbe interaction can further lead to the development of biofertilizers to improve soil productivity in a disturbed ecosystem, like mine soil dumps.