Cloning and characterization of HindIII fragments of bovine herpesvirus-1 DNA from an Indian isolate

摘要

HindIII fragments of DNA from an Indian isolate of bovine herpesvirus-1 were cloned in pUC9 plasmid vector. Five of the fifteen fragments could be cloned by shotgun method and identified by their size and digestion with other restriction enzymes. The largest fragment of about 12.0 kbp was identified as HindIII fragment 'G' and the smallest fragment was HindIII fragment 'O' of 0.4 kbp. Fragments could be further characterized and partially mapped for other restriction enzyme sites.