Dual-molecule spectroscopy: molecular rulers for the study of biological macromolecules

摘要

Single-molecule detection and spectroscopy by laser induced fluorescence offer many applications to life sciences. We review recent techniques and experiments based on the detection and spectroscopy of two near-by molecules. The spectroscopic signature of the light emitted from the two molecules can be used to measure the distance, orientation and relative dynamics between biological macromolecules.