The Role of A. thaliana MicroRNA-Regulated Protein-Protein Interaction Networks in Plant-Pathogen Interaction Study

摘要

In this study, the microarray data for Arabidopsis thaliana infected with Xanthomonas campestris pv. campestris (Xcc) is analyzed, where differentially expressed genes (DEGs) are identified, and Gene Set Enrichment Analysis (GSEA) are employed for functional study. As a result, highly relevant pathogen resistant pathways are inferred. The analyses suggested that certain proteins, i.e., SGT1, HSP and SEC, and secondary metabolites are actively involved in plant defense mechanism. Furthermore, the results of DEGs for various different conditions; such as, infection by different strains of Agrobacterium tumefaciens, are determined. Furthermore, protein-protein interaction (PPI) plays an important role in host-pathogen interaction (HPI). Gene Ontology (GO) annotation for microRNA-regulated PPI, pathogen resistant genes and transcription factors information are implemented, such resources can provide new insights for microRNA-regulated PPI networks in HPI study. In addition, microRNA-regulated PPI networks are clustered by using the k-means algorithm, and the results are further analyzed by GSEA. It was found that the hormone mediated signaling pathway plays an essential role in HPI.