A SENSITIVE METHOD FOR THE DETERMINATION OF BROMINATED DIPHENYL ETHER (BDE-209) IN WATER BASED ON DISPERSIVE LIQUID-LIQUID MICROEXTRACTION AND ORTHOGONAL TEST

摘要

The determination of brominated diphenyl ether (BDE-209) in water samples was investigated using orthogonal test (L_(27) (3)~(13)), based on dispersive liquid-liquid microex-traction (DLLME) combined with high-performance liquid chromatography (HPLC). The results indicated that the volume of extraction solvent and ionic strength were the main parameters to influence enrichment factor (EF) and extraction recovery (ER) of BDE-209. The two parameters were optimized on the basis of single factor test. Under optimum conditions (20 μ1 of tetrachloromethane, 1.0 ml of acetonitrile, pH 7, 1% of NaCl, extraction time of 5 min), EF and ER were 541 and 92.66%, respectively. The calibration graph was linear in the range of 0.1-100 ng ml~(-1) (r~2= 0.9999) and relative standard deviation (RSD) was 3.8-13.0%. The limits of detection (LOD) and quantification (LOQ) were 7.7 and 52.63 pg ml~(-1), respectively. This method had a higher EF than the published DLLME for the determination of PBDEs. Additionally, LOD of this method was lower by one order of magnitude than those of other methods with HPLC detection based on the optimum results of the extraction conditions. The proposed method was also proved to be applicable for the analysis of BDE-209 in real environmental water samples.