BACTERIOPLANKTON COMMUNITY COMPOSITION AND THE INFLUENCE OF WATER CHEMISTRY IN SMALL LAKES OF NANJING CITY, CHINA

摘要

The spatial distributions of bacterioplankton communities among different lakes were determined in three eu-trophic lakes (Lake Pipa, Lake Qianhu and Lake Yueya) of Nanjing City, China. The bacterial community composition was investigated using denaturing gradient gel electropho-resis (DGGE). The results indicated that bacterial composition clustered by the individual lakes. Three 16S rRNA clone libraries were constructed and analyzed to demonstrate the differences in bacterial community structure among the different lakes. Spatial variability was found among the DGGE banding patterns, and this variability was supported by the analysis of similarity (ANOSIM). Cyanobacteria were the most dominant taxa in Lake Pipa and Lake Yueya, comprising 86.4% and 66% of the clone libraries, respectively, whereas P-proteobacteria was most abundant in Lake Qianhu, accounting for 41.7% of the clone library. The results data were further analyzed by canonical correspondence analysis (CCA), which revealed that bacterioplankton community composition was significantly correlated with total nitrogen (TN).