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首页> 外文期刊>Food Chemistry >Development and application of recombinant antibody-based immunoassays to tetraconazole residue analysis in fruit juices
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Development and application of recombinant antibody-based immunoassays to tetraconazole residue analysis in fruit juices

机译:基于重组抗体的免疫分析技术在果汁中四康唑残留分析中的应用

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摘要

Tetraconazole is currently used as a fungicide in fruit and vegetables. The aim of this work was the development of immunochemical techniques based on recombinant antibodies for the screening of tetraconazole residues in fruit juices. Recombinant antibodies were produced from a hybridoma cell line secreting a monoclonal antibody specific for tetraconazole and from lymphocytes of mice hyperimmunised with tetraconazole haptens conjugated to bovine serum albumin. From these antibodies, enzyme-linked immunosorbent assays in the conjugate-coated format were developed, which were able to detect tetraconazole standards down to 1 ng/mL. From recovery studies with spiked samples, these immunoassays determined tetraconazole in orange and apple juices with acceptable reproducibility (coefficients of variation below 25%) and recoveries (ranging from 78% to 145%) for a screening technique. The analytical performance of RAb-based immunoassays was fairly similar to that of the MAb-based immunoassays. Due to their simplicity and high sample throughput, the developed recombinant-based immunoassays can be valuable analytical tools for the screening of tetraconazole residues in fruit juices at regulatory levels.
机译:四康唑目前在水果和蔬菜中用作杀菌剂。这项工作的目的是开发基于重组抗体的免疫化学技术,用于筛选果汁中的四康唑残留。从分泌对四康唑具有特异性的单克隆抗体的杂交瘤细胞系和由与牛血清白蛋白缀合的四康唑半抗原超免疫的小鼠的淋巴细胞产生重组抗体。从这些抗体中,开发出了缀合物包被形式的酶联免疫吸附测定,能够检测低至1 ng / mL的四康唑标准品。通过对加标样品的回收率研究,这些免疫测定法确定了橙汁和苹果汁中的四康唑具有可重复性(变异系数低于25%)和筛查技术的回收率(从78%至145%)。基于RAb的免疫测定的分析性能与基于MAb的免疫测定的分析性能相当相似。由于其简单性和高样品通量,开发的基于重组的免疫测定法可以成为有价值的分析工具,可用于以监管水平筛查果汁中的四康唑残留物。

著录项

  • 来源
    《Food Chemistry》 |2014年第15期|205-213|共9页
  • 作者单位

    Instituto lnteruniversitario de Investigation en Bioingenieria y Tecnologia Orientada al Ser Humano, Universitat Politecnica de Valencia, Camino de Vera s, Edificio 8B-N, 46022 Valentia, Spain;

    Instituto lnteruniversitario de Investigation en Bioingenieria y Tecnologia Orientada al Ser Humano, Universitat Politecnica de Valencia, Camino de Vera s, Edificio 8B-N, 46022 Valentia, Spain;

    Instituto lnteruniversitario de Investigation en Bioingenieria y Tecnologia Orientada al Ser Humano, Universitat Politecnica de Valencia, Camino de Vera s, Edificio 8B-N, 46022 Valentia, Spain;

    Instituto lnteruniversitario de Investigation en Bioingenieria y Tecnologia Orientada al Ser Humano, Universitat Politecnica de Valencia, Camino de Vera s, Edificio 8B-N, 46022 Valentia, Spain;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Immunochemical analysis; Recombinant antibodies; ScFv; ELISA; Tetraconazole; Fungicide residues; Fruit juices;

    机译:免疫化学分析;重组抗体;ScFv;ELISA;四康唑;杀菌剂残留;水果汁;

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