Authentication of Thailand jasmine rice using RAPD and SCAR methods

摘要

A protocol for jasmine rice authentication was established in order to determine whether a Thai jasmine rice product meets legal requirement. Both RAPD and SCAR methods were used on the basis of previous study of jasmine-rice specific genome characteristics. Two RAPD markers, RI2-449 and RI5-1107, were selected and SCAR primer pairs were designed for RI5-1107. Twenty-four rice cultivars, which represented typical jasmine rice and non-jasmine rice were analyzed to confirm the specificity and reproducibility of DNA method. The purity of jasmine rice was calculated by analyzing every single kernel from test sample. Five in-house prepared samples were quantified to prove the accuracy of DNA method, and 15 imported samples were analyzed by DNA method as well as physical/chemical method to confirm the consistency between DNA method and traditional method. Based on the standard deviation obtained by comparing DNA method and traditional method, a testing threshold for jasmine rice was decided. Investigation of eight commercial samples from market showed that 75% products meet the requirement.