Analysis of hydrogen peroxide in cigarette smoke from selected Chinese cigarette brands under conventional and intense machine smoking conditions

摘要

The purpose of the present work was to investigate the concentration ranges of H2O2 generated from cigarette smoke of selected Chinese brands and the effect of different smoking behaviors on the deliveries of H2O2. A simple, rapid and reliable fluorescence assay was developed for the measurement of H2O2 based on the catalytic activity of hemoglobin using 10-acetyl-3, 7-dihydroxyphenoxazine (Amplex Red) as the substrate. The proposed method was applied to determine the H2O2 deliveries from mainstream cigarette smoke of 30 Chinese (5 blended and 25 Virginia) and two reference cigarettes (3R4F and 1R5F) under two machine smoking regimes. Results showed that, under ISO smoking conditions, Chinese blended cigarettes had lower H2O2 yields than Chinese flue-cured cigarettes (mean value 2.41 vs. 4.69 μg cig−1, p 0.05). As expected, the deliveries of H2O2 for all cigarettes analyzed were higher under Canadian intense smoking conditions than those under ISO conditions (ISO mean 4.31 μg cig−1 vs. Canadian mean 5.96 μg cig−1, p 0.05). In addition, the ratios of H2O2 to nicotine yields were utilized to show the relative differences in yields among brands tested in this work and more than a twofold difference was found for most of selected Chinese blended and flue-cured cigarettes.