Effect of the fungicide clotrimazole on the bioconcentration of benzo[a]pyrene in gizzard shad (dorosoma cepedianum): in vivo and in vitro inhibition of cytochrome P4501A activity

摘要

Clotrimazole was found to inhibit in vivo and in vitro hepatic microsomal ethoxyresorufin O-deethylase (EROD) activity in gizzard shad (Dorosoma cepedianum). Gizzard shad pretreated with 50 mg clotrimazole/kg and then exposed for 1 or 3 d to benzo[a]pyrene (BaP)(0.68 μg/L) had significantly lower EROD activity compared to fish that were exposed to BaP alone. Following 1 and 3 d of BaP exposure, groups pretreated with clotrimazole had a 14- and 4-fold decrease in EROD activity and had biocon- centrated 8 and 11 times more parent BaP, respectively, compared to groups exposed to BaP alone.