METABOLISM OF FLUORANTHENE IN DIFFERENT PLANT CELL CULTURES AND INTACT PLANTS

摘要

The metabolism of fluoranthene was investigated in ll cell cultures of different plant species using a [~(14)C]-labeled standard. Most species metabolized less than 5 of fluoranthene to soluble metabolites and formed less than 5 nonextractable residues during the standardized 48-h test procedure. Higher metabolic rates were observed in lettuce (Lactuca sativa, 6), wheat (Tricitum aestivum, 9), and tomato (Lycopersicon esculentum, l5). A special high metabolic rate of nearly 50 was determined for the rose species Paul's Scarlet. Chromatographic analysis of metabolites extracted from aseptically grown tomato plants proved that the metabolites detected in the cell cultures were also formed in the intact plants. Metabolites produced in tomato and rose cells from [~(14)C]-fluoranthene were conjugated with glucose, glucuronic acid, and other cell components. After acid hydrolyses, the main metabolite of both species was l-hydroxyfluoranthene as identified by gas chromatography-mass spectrometry and high- performance liquid chromatography with diode array detection. The second metabolite formed by both species was 8-hydroxy- fluoranthene. A third metabolite in tomatoes was 3-hydroxyfluoranthene.