SEPARATION AND CHARACTERIZATION OF GALL BLADDER BILE METABOLITES FROM SPECKLED TROUT, SALVELINUS FONTINALIS, EXPOSED TO INDIVIDUAL POLYCYCLIC AROMATIC COMPOUNDS

摘要

Speckled trout, Salvelinus fontinalis, were orally exposed to individual polycyclic aromatic compounds(PACs)rep- resented by benzo［a］phrene, chrysene, dibenzofuran, dibenzothiophene, fluorene, phenanthrene, and pyrene. Fish were sacrificed 7 d after exposure and the gall bladder removed for bile analysis. High pressure liquid chromatogrpahy(HPLC)with fluorescence(F)and ultraviolet(UV)detection was used to determine the presence of PAC derivatives in the bile without pretreatment. Glucuronide conjugates were predominant in all exposures with variable amounts(0-53/100)of phenols and starting material.