DEVELOPMENT OF FLOW CYTOMETRY-BASED ALGAL BIOASSAYS FOR ASSESSING TOXICITY OF COPPER IN NATURAL WATERS

摘要

Copper toxicity to the freshwater algae Selenastrum capricornutum and Chlorella sp. And the marine algae Phaeodac- tylum triconutum and Dunaliella tertiolecta was investigated using different measured by flow cytometry: cell division rate inhibition, chlorphyll a fluorescence, cell size(i.e., light-scattering), and enzyme activity. These parameters were assessed regarding their usefulness as alternative endpoints for acute(1-24 h)and chronic(48-72 h)toxicity tests. At copper concentrations of 10μg/L or less, significant inhibition(50/100)of the cell division rate was observed after 48-and 72-h exposures for Chlorella sp., S. Capricornutum, and P. tricornutum.