Evaluation of physiological changes induced by the fluoroquinolone antibiotic ciprofloxacin in the freshwater macrophyte species Lemna minor and Lemna gibba

摘要

The worldwide increase in the consumption of antibiotics is becoming a concern for the scientific community, since the presence of their residues in the wild poses specific challenges, especially in ecotoxicological terms. Currently, antibiotics are used for a wide range of purposes, being used against bacterial diseases but also as growth promoters. As a result, their environmental presence can affect wild organisms, especially those from the aquatic environment. This scenario leads to the need of characterizing the toxicity of antibiotics, especially towards non-target organisms. In this study we selected two species of aquatic macrophytes, Lemna minor and Lemna gibba, which are standard plant species inscribed in ecotoxicological testing guidelines. In this work we characterized the toxic effects of the quinolone antibiotic ciprofloxacin (in levels of 0.005, 0.013, 0.031, 0.078, and 0.195 mg/L), focusing on its potential toxicity towards photosynthetic mechanisms, and pro-oxidant effects. These objectives were attained by measuring the concentrations of chlorophyll a and b, and carotenoids levels. The determination of the quantum yield allowed assessing the effects of ciprofloxacin on the photochemical efficiency of the Photosystem II (PSII). The pro-oxidant effects induced by ciprofloxacin were evaluated by measuring oxidative stress biomarkers, such as catalase activity, and also by determining lipoperoxidation levels. The obtained results showed no differences in terms of the content of both chlorophylls a and b, or any change in the photochemical efficiency of the PSII; however, the global carotenoids content of L. gibba were significantly decreased. The activity of the anti-oxidant enzyme catalase was also significantly increased in L. minor. L. gibba showed a decrease in lipid peroxidation levels, but only for the two lowest concentrations of ciprofloxacin. The global set of data shows the activation of the anti-oxidant defensive system of both plant species, a response that was likely activated by the pro-oxidant character of ciprofloxacin. Our data demonstrate the interference of this therapeutic compound at different levels of plant metabolism, at ecologically relevant concentrations. In fact, the obtained results are of ecological relevance since they illustrate deleterious effects that may compromise the physiology of aquatic non-target plant species.