Specific accumulation of CYP94A1 transcripts after exposure to gaseous benzaldehyde: Induction of lauric acid oo-hydroxylase activity in Vicia sativa exposed to atmospheric pollutants

摘要

The effects of air pollutants such as aldehydes, ozone, nitrogen dioxide and benzene on fatty acid o> hydroxylase activity in Vicia sativa microsomes have been investigated. Four days old etiolated V. sativa seedlings were exposed to different concentrations of selected pollutants for varying exposure times. Growing etiolated V. sativa seedlings in air containing the gaseous benzaldehyde (150 nM) led to an 8-fold enhancement of lauric acid co-hydroxylase activity in microsomes of treated plants compared to controls grown in pure air (96 + 10 versus 12 + 2 pmol/min/mg protein, respectively). The induction increased with increasing gas phase concentrations (10-1300 nM) and the maximum of activity was measured after 48 h of exposure. Northern blot analysis revealed that this induction occurred via transcriptional activation of the gene coding for CYP94A1. The absence of CYP94A2 and CYP94A3 transcription activation together with the missing effect on epoxide hydrolases activities indicate the specificity of CYP94A1 induction by benzaldehyde. Exposure to nitrogen dioxide, ozone and formaldehyde also stimulated lauric acid co-hydroxylases activity while exposure to benzene did not show any effect.