Relative Phytoplankton growth responses to physically and chemically dispersed South Louisiana sweet crude oil

摘要

We conducted controlled laboratory exposure experiments to assess the toxic effects of water-accommodated fractions (WAFs) of South Louisiana sweet crude oil on five phytoplankton species isolated from the Gulf of Mexico. Experiments were conducted with individual and combinations of the five phyto-plankton species to determine growth inhibitions to eight total petroleum hydrocarbon (TPH) equivalent concentrations ranging from 461 to 7,205 ppb. The composition and concentration of crude oil were altered by physical and chemical processes and used to help evaluate crude oil toxicity. The impact of crude oil exposure on phytoplankton growth varied with the con-centration of crude oil, species of microalgae, and their community composition. At a concentration of TPH ＜ 1,200 ppb, dinoflagellate species showed significantly better tolerance, while diatom species showed a higher tolerance to crude oil at higher concentrations of TPH. For both groups, the larger species were more tolerant to crude oil than smaller ones. The toxicity potential of crude oil seems to be strongly influenced by the con-centration of polycyclic aromatic hydrocarbons (PAHs). The addition of the dispersant, Corexit~® EC9500A, increased the amount of crude oil up to 50-fold in the water column, while the physical enhancement (vigor-ous mixing of water column) did not significantly in-crease the amount of TPH concentration in the water column. The species response to crude oil was also examined in the five-species community. Each phyto-plankton species showed considerably less tolerance to crude oil in the five-species community compared to their individual responses. This study provides baseline information about individual phytoplankton responses to crude oil and dispersed crude oil for subsequent research efforts seeking to understand the impacts of oil on the phytoplankton in the bigger picture.