A modified toxicity testing method using tropical marine microalgae

摘要

Toxicity testing of four heavy metal s9Cd, Cu, Mn and As) using four species of tropical marine phytoplankton, Chaetoceros calcitrans, Isochrysis galbana, tetraselmis tetrahele and Tet- raselmis sp., was carried out in multiwell plates with test volumes of 2 mL and the results compared to those of standard, large volume, shake-flasks. IC_50 values (concentrations of metals estimated to inhibit 50/100 growth relative to the control) were determined after 96 hours based on automated O.D. readings measured in Elisa microplates by a Multiskan spectrophotometer.