EFFECT OF POTASSIUM DICHROMATE AND FENITROTHION ON HEMOGLOBINS OF CHIRONOMUS RIPARIUS MG. (DIPTERA, CHIRONOMIDAE) LARVAE: POTENTIAL BIOMARKER OF ENVIRONMENTAL MONITORING

摘要

In an attempt to identify Chironomus hemoglobins as biomarkers for environmental monitoring, alterations in the hemoglobins in Chironomus riparius Mg. (Diptera: Chironomidae) larvae, exposed to potassium dichromate and fenitrothion, were investigated under laboratory conditions. The hemoglobins were evaluated in terms of their total contents by a cyanomethemoglobin procedure, individual components by electrophoresis of isoelectric focusing, and their oxidation by multi wavelength rapid-scanning spectrophotometry. The total hemoglobin contents increased at the high level of fenitrothion exposure. No variations in the individual hemoglobin component levels were found, by exposure to either fenitrothion or potassium dichromate. Whereas, the absorption spectra of the hemoglobins showed decreases in the peaks corresponding to the oxyhemoglobins by exposure to both compounds, but more sensitively by the chromium, which probably reflects the increase of the autoxidation of the oxyhemoglobins to methemoglobins by these compounds. These results suggest that autoxidation of the hemoglobins in Chironomus riparius seems to be a sensitive parameter in response to redox-active chemical exposure, and this biochemical parameter could be developed as a biomarker in environmental monitoring.