Distinct Oxidative Damage of Biomolecules by Arrays of Metals Mobilized from Different Types of Airborne Particulate Matters; SRM1648, Fine (PM_(2.5)), and Coarse (PM_(10)) Fractions

摘要

This study was performed to examine the in vitro toxicities which are incurred due to the mobilization metals from standard reference material (SRM) 1648, fine (PM_(2.5)), and coarse (PM_(10)) particulate matter collected in Seoul metropolitan area. DNA single strand breaks of approximately 74% and 62% for PM_(2.5) and for PM_(10), respectively, were observed in the presence of chelator (EDTA or citrate)/ reductant (ascorbate), as compared to the control by 2% without chelator or reductant. PM_(2.5) induced about 40% more carbonyl formation with proteins in the presence of EDTA/ascorbate than PM_(10). Therefore, more damage to biomolecules was incurred upon exposure to PM_(2.5) than to PM_(10). The treatment of a specific chelator, desferrioxamine, to the reaction mixture containing chelator plus reductant decreased the extent of damage to DNA to the level of the control, but did not substantially decrease the extent of damage to proteins. This suggests that different arrays of metals were involved in the oxidation of DNA and proteins.