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首页> 外文期刊>Journal of Virology >Association of Epstein-Barr virus early antigen diffuse component and virus-specified DNA polymerase activity.
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Association of Epstein-Barr virus early antigen diffuse component and virus-specified DNA polymerase activity.

机译:Epstein-Barr病毒早期抗原弥漫性组分和病毒指定的DNA聚合酶活性的关联。

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摘要

The role of Epstein-Barr virus (EBV) early antigen diffuse component (EA-D) and its relationship with EBV DNA polymerase in EBV genome-carrying cells are unclear, EBV-specified DNA polymerase was purified in a sequential manner from Raji cells treated with phorbol-12,13-dibutyrate and n-butyrate by phosphocellulose, DEAE-cellulose, double-stranded DNA-cellulose, and blue Sepharose column chromatography. Four polypeptides with molecular masses of 110,000, 100,000, 55,000, and 49,000 daltons were found to be associated with EBV-specified DNA polymerase activity. A monoclonal antibody which could neutralize the EBV DNA polymerase activity was prepared and found to recognize 55,000- and 49,000-dalton polypeptides. An EA-D monoclonal antibody, R3 (G. R. Pearson, V. Vorman, B. Chase, T. Sculley, M. Hummel, and E. Kieff, J. Virol. 47:183-201, 1983), was also able to recognize these same two polypeptides associated with EBV DNA polymerase activity. It was concluded that EBV EA-D polypeptides, as identified by R3 monoclonal antibody, are critical components of EBV DNA polymerase.
机译:Epstein-BART病毒(EBV)早期抗原弥漫性组分(EA-D)的作用及其与EBV DNA聚合酶在EBV基因组携带电池中的关系尚不清楚,以依次纯化EBV指定的DNA聚合酶从治疗的Raji细胞纯化通过磷纤维素,DEAE-纤维素,双链DNA-纤维素和蓝琼脂糖柱色谱法与菲尔伯-12,13-二丁酸酯和正丁酸丁酸酯。发现具有110,000,100,000,55,000和49,000道尔顿的分子量为110,000,100,000,55,000和49,000道尔顿的四种多肽与EBV指定的DNA聚合酶活性有关。制备可以中和EBV DNA聚合酶活性的单克隆抗体,发现识别55,000-和49,000-道顿多肽。 EA-D单克隆抗体,R3(GR Pearson,V.Vorman,B. Chase,T.Sculley,M. Hummel和E. Kieff,J.Vireol。47:183-201,1983),也能够识别与EBV DNA聚合酶活性相关的相同两种多肽。得出结论,由R3单克隆抗体鉴定的EBV EA-D多肽是EBV DNA聚合酶的关键组分。

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