Probing size characteristics of disinfection by-products precursors during the bioavailability study of soluble microbial products using ultrafiltration fractionation

摘要

Soluble microbial products (SMPs) discharged into surface water may increase the formation of disinfection byproducts (DBPs) in downstream drinking water treatment plants. In this study, ultrafiltration (UF) fractionation was used to separate SMPs into homogenous components. An aerobic microbial experiment was conducted to evaluate the bioavailability of individual molecular weight (MW) fractions of SMPs in surface water and the impact on their DBP formation, facilitating the interpretation of SMPs characterization and DBPs reactivity. For SMPs, organics with MW 1 kDa were the primary fraction, containing the most abundant humic substances. The 30 kDa MW 100 kDa fraction was the lowest in SMPs but had the highest SUVA values. After biodegradation, the bioavailability of physical fractions increased with the increasing MW size. However, the SUVA value, except for MW 1 kDa, increased in individual fraction after biodegradation. Low molecular weight SMPs fractions (MW 10 kDa) were major precursors for DBP in which trichloromethane (TCM) was the most abundant. The 10 kDa MW 100 kDa fractions were found to be more active in formation of chloral hydrate (CH), and MW 100KDa had relative abundant dichloroacetonitrile (DCAN) formation. After biodegradation, TCM precursors with MW 1 kDa were removed by approximately 20%, whereas the increase of TCM formation was observed in 1 kDa MW 100 kDa fraction. CH formation from 1 kDa MW 10 kDa increased considerably, but those from 10 kDa MW 30 kDa decreased after biodegradation, as a result of the biotransformation of large organic acids to small organic acids. In terms of DBP reactivity, the TCM yield for the MW 1 kDa fraction had no significant change while the 30 kDa MW 100 kDa fraction exhibited the greatest increase (approximately 8 times) in TCM yield.