Bioanalytical Fluorescent Reagents Based on Polyacrolein-Containing Particles Labeled with Semiconductor CdSe/ZnS Nanocrystals

摘要

The majority of modern analytical methods in biology, biotechnology, and medicine are based on the use of fluorescent labels. Today, semiconductor nanocrystals, also known as quantum dots (QDs), draw attention of researchers as promising fluorophores. An example of such quantum dots is CdSe/ZnS-nanocrystals. In comparison with organic fluorescent dyes, QDs have many advantages, such as a high quantum yield, excellent photostability, a narrow fluorescence peak, and a broad excitation spectrum, which allows using the same radiation source for QDs of all sizes. The unique property of QDs is the possibility to regulate the spectral range of fluorescence by controlling the size and chemical composition of QDs. However, the difficulty of obtaining aqueous suspensions of QDs and their insufficient chemical and colloidal stability and photostability, as well as the absence of surface functional groups significantly limit their use in bio-analysis [1,2].