Cellular Immune Responses Against Proinsulin No Evidence for Enhanced Reactivity in Individuals With IDDM

摘要

Investigations of humans and nonobese diabetic mice suggest that proinsulin and/or fragment of the region spanning C-peptide and the B-chain of insulin (i.e., proinsulin peptide) may serve as key autoantigens in IDDM. Therefore, we analyzed cellular immune reac- Tivities against these molecules in people with or at Varying risks for the disease to clarify their role in the Pathogenesis of IDDM. In vitro peripheral blood Mononuclear cell (PBMC) responses against these anti- Gens, a control antigen (tetanus toxoid), and phyto- Hemaglutinin were determined in 60 individuals with Newly diagnosed IDDM (≤1 day from diagnosis) in 34 Islet cell cycoplasmic autoantibody- and/or insulin Autoantibody-negative first-degree relatives of the IDDM subjects, and in 28 autoantibody-negative control Subjects.