Nerve growth factor increases insulin secretion and barium current in pancreaticβ-cells

摘要

We analyzed the effect of a brief exposure to nerve growth factor(NGF)on insulin secretion and macro- scopic barium currents of single adult rat pancreatic β-cells. After a 1-h exposure to NGF(50 ng/ml), single β-cells show a 2.5-fold increase in the insulin secretion index in 5.6 mmol/l glucose and a nearly twofold in- crease in 15.6 mmol/l glucose compared with control cells. We have recently demonstrated that pancreatic β-cells synthesize and secrete NGF.