Comparative study of growth and biochemical attributes of Avicennia marina (Forssk.) Vierh of Indus delta and in vitro raised plants established at Jamshoro, Sindh Pakistan

摘要

The study was conducted to optimize medium and growth conditions for micropropagation and field establishment of grey mangrove, Avicennia marina (Forssk.) Vierh using nodular stem sections as explants. Varying concentrations of 6- benzylaminopurine (BAP), kinetin (Kin) and indole-3-acetic acid (IAA) were supplemented in medium to assess their effects on the development of microshoots while indole-3-butyric acid (IBA), 1-naphthalene acetic acid (NAA) and indole-3-acetic acid (IAA) were supplemented to assess their role in root formation. All the treatments promoted shoot formation, the highest percentage of explants (86%) formed microshoots from the axillary buds with the highest number of microshoots per explant (2.4?0.1) were noted on MS medium consisting of 0.5mg/L of BAP, 1.0mg/L of Kin and 0.25mg/L IAA. In contrast 1.0mg/L IBA containing medium caused root formation in the maximum microshoots (82%) with 2.16?0.05 roots per microshoot, 27.5?0.5mm average root length. Furthermore, 65% of micropropagated A. marina plants were successfully survived for two years during 2015-17 in region II (Jamshoro). These plants showed better growth as plant height, numbers of leaves, chlorophyll contents, sugars and proteins were increased comparing with same age plants of region I. On the other hand, an increase in stem diameter, number of branches, and the amount of total phenolic contents, total flavonoids and antioxidants was found in plants of region I (Shah Bander, Indus Delta). In conclusion, micropropagated plantlets of A. marina were successfully established in geographically hot region which will support to establish mangrove plants around the banks of Indus River.