IDENTIFICATION AND DOCUMENTATION OF EDIBLE INSECTS OF VESPA AFFINIS IN NAGALAND BY USING DNA BARCODE TECHNIQUES

摘要

DNA bar-coding is an ordered strategy that utilizes a little genetic marker in insect DNA to distinguish animal categories, as well as unidentified species. The present investigation manages the utilization of COI gene sequences in the distinguishing proof and documentation of edible insects in Nagaland. More than 2000 types of edible insects are recorded in more than 300 ethnic groups all around the world. It is evaluated that more than 200 types of species of edible insects and other invertebrates are consumed in North East India. In the preliminary study, Vespa affinis was selected and morphologically identified with the help of local tribals in Nagaland. Vespa affinis was identified by using phylogenetic tree analysis, and COI gene sequences were submitted in NCBI to received the accession number (MN820554), BOLD (ACH6486), and COI gene sequence were submitted in BOLD to generated DNA barcode for edible insects of Vespa affinis.