Comparison of traditional and modern methods for identification of Plasmodium species

摘要

If we think about one of the dreaded endemic in the last century, undoubtedly Malaria name comes at the top. In case of infectious disorders, it is very well established fact that earlier the diagnosis of infectious disease, lesser are the chance to spread of such a disease in public domain and subsequently lesser the cascading effect. Still due to the limitations of testing a few no of cases by a clinical laboratory or research entity is also a big hinderance to give accurate results. There are many traditional methods for Plasmodium vivax identification and common in use are Giemsa staining (thick and thin) and Fluoroscence microscopic malarial diagnostic test. In spite of the fact that ibid mentioned methods are effective in diagnosis of Plasmodium vivax yet it was observed that both the methods in one or other way are time consuming and labour intensive. Therefore, one more method came into existence which has the potential to rapidly diagnose the parasite is known as Non microscopic Rapid Diagnostic test (RDT). RDT uses single step immunochromatography assay to identify the Plasmodium vivax. RDT is simple to execute, more convenient, provide fast diagnosis and less labour intensive as compared to the former two. Aim of the current work is to find out the authenticity, precision level of various techniques to identify the malarial parasite and find out which one is more economical, easy to operate and accurate. A total of 104 patients were taken in study who were suffering from malaria caused by Plasmodium vivax. Out of the them, 85 patients were positive by using technique of Flouroscence Microscopic test while a total of 94 patients were positive by using technique of Giemsa staining, On the other hand Non Microscopic Rapid Diagnostic test shown a total of 101 patients positive for malaria.