In silico genomic and proteomic analyses of three heat shock proteins (HSP70, HSP90-α, and HSP90-β) in even-toed ungulates

摘要

BackgroundHeat shock proteins (HSPs) play important roles in the responses to different environmental stresses. In this study, the genomic and proteomic characteristics of three HSPs (HSP70,HSP90-αandHSP90-β) in five even-toed ungulates (sheep, goats, water buffalo, Zebu cattle and cattle) were analyzed using Multiple sequence alignment, SWISS modeling and phylogenetics analysis tools.ResultsThe bioinformatic analysis revealed that theHSP70gene in cattle, Zebu cattle, and goat is located on chromosome 23, and is intronless, while in water buffalo and sheep it is located on chromosomes 2 and 20, respectively, and contains two exons linked by one intron. TheHSP90-αgene is located on chromosome 21 in cattle, Zebu cattle, and goat, while in water buffalo and sheep it is located on chromosomes 20 and 18, respectively. TheHSP90-βgene is located on the same chromosome as theHSP70gene and contains 12 exons interspersed by 11 introns in all studied animals.In silicoExpasy translate tool analysis revealed thatHSP70,HSP90-αandHSP90-βencode 641, 733, and 724 amino acids, respectively. The data revealed that goatHSP70protein has seven variable amino acid residues, while in both sheep and cattle only one such amino acid was detected.ConclusionsThis study will be supportive in providing new insights into HSPs for adaptive machinery in these studied animals and selection of target genes for molecular adaptation of livestock.How to cite:Raza SHA, Hassanin AA, Dhshan AIM, et al.In silicogenomic and proteomic analyses of three heat shock proteins (HSP70, HSP90-α, and HSP90-β) in even-toed ungulates. Electron J Biotechnol 2021;53. https://doi.org/10.1016/j.ejbt.2021.07.002.