Electrochemical Biosensors Based on Carbon Nanocages for the Detection of NADH and Ethanol

摘要

Carbon nanocages (CNCs) were used to prepare modified electrodes and showed excellent electrocatalytic activities in electrochemical catalysis for reduced nicotinamide adenine dinucleotide (NADH). The CNCs-modified glassy carbon electrode (CNCs/GCE) has a high sensitivity to the measurement of NADH (15.78 μA·μM-1 ·cm-2 ) and gave a low detection limit of 0.34 μM. An amperometric ethanol biosensor was prepared by combining alcohol dehydrogenase (ADH) with CNCs/GCE. The ethanol biosensor exhibited a wide linear range up to 5 mM with a relatively low detection limit of 0.30 mM as well as a high sensitivity of 10.85 nA/mM without suffering any interference from some common electroactive compounds.