Effect of Solvent Flow Rate in Mini-Column Testing of 99mTc-Mertiatide

摘要

>Objective: The recommended method for radiochemical purity testing of 99mTc-mertiatide involves the use of a C-18 solid-phase mini-column cartridge. The mertiatide package insert states that the solvents should be a€?pushed through the cartridge slowly,a€? but a flow rate is not specified. The mini-column cartridge instruction sheet recommends flow rates of 5-10 and 2-10 mL/min for conditioning and for elution, respectively, of the cartridge. The purpose of this study was to evaluate the effect of different flow rates on determining the radiochemical purity of 99mTc-mertiatide.