STILBINOID COMPOUND FROM ETHANOL EXTRACT OF THE BARK ‘RARU’, Vatica pauciflora BLUME (DIPTEROCARPACEAE)

摘要

Vatica pauciflora Blume (local name: Raru) is known by Tapanuli’s people widely as additional ingredients mixed to make toddy beverage as tuak which made from nira (sugar palm juice).This addition is intended to match the sweet taste and alcohol.The local people also know raru bark is used as diabetic treatment.In this research, isolation of chemical compound based on cytotoxic treatment with Brine Shrimp Lethality Assay (BSLA) of ethanol extract raru’s bark.Separation process was conducted by column chromatography [(SiO2; i.n-hexane-ethylacetate = 1 : 1; ii.CHCl3- MeOH = 10 : 1 ~ 1 : 1; iii.CHCl3-MeOH-H2O = 5 : 5 : 1; iv.CHCl3-MeOH = 1 : 1 and further purification with preparative TLC (SiO2; CHCl3-MeOH: H2O = 7: 3: 1 and gave one pure isolate with LC50 of 34.20 ppm.Based on the interpretation of Ultra Violet (UV), FT-Infra Red (FT-IR) and Nuclear Magnetic Resonance (1 H & 13C-NMR) spectra show that the compound is a stilbinoid, piceid acid.