Ureaplasma diversum and Ureaplasma sp. in nasal cavity of pigs: Distribution among herds and individual frequency based on pooled samples

摘要

The role ofUreaplasma diversumin the porcine respiratory disease complex (PRDC), its circulation among herds and prevalence in live pigs is unknown; thus, the objectives of this study were: to determine the presence ofU. diversumin indoor intensive pig herds and to determine the individual frequency of pigs withU. diversumfrom pooled samples. A cross sectional study was carried out in 16 indoor intensive herds from Córdoba and La Pampa provinces, collecting eight nasal swabs specimens that were further processed by two pools of four specimens each by a PCR targeting a fragment of 16S ribosomal RNA gene. Four PCR products were sequenced and aligned against data bases.U. diversum–Ureaplasmasp. were detected in 56.3% of the analyzed herds, with 16.5% (95% CI 8.2–30.8) of positive pigs. It was concluded thatU. diversum–Ureaplasmasp. are present in the nasal cavity of live pigs, being widely distributed among herds.